Biochemistry and molecular biology laboratory course

生物化学与分子生物学实验教程 英文版

Price: $18.00


Author: Yuefei-Xu & Yuxiang-Tian
Language: English
ISBN/ISSN: 9787030418968
Published on: 2014-09

《生物化学与分子生物学实验教程 Biochemistry and molecular biology laboratory course(英文版)》针对生物化学教学及研究中涉及的三类主要的生物分子——蛋白质、酶和核酸,根据生物大分子的理伦性质,结合实用的电泳技术、层次技术、离心技术、分子克隆技术等,综合系统地介绍生物大分子的分离、纯化、含量测定、分析鉴定和应用,并结合教学中物质代谢、酶促反应动力学的系统分析,设计实验并融入相关实验技术和研究新进展,使学生在实验教学中掌握知识、技能的同时,体会对科研方法的选择与评价。

Part 1 Basic techniques of biochemistry and molecular biology 
Chapter 1 Basic techniques of preparation of biomacromolecules 
Section 1 Salting out 
Section 2 Dialysis and ultrafiltration 
Chapter 2 Spectrophotometry 
Section 1 Basic principles 
Section 2 The basic construction of spectrophotometers 
Section 3 Some home—made spectrophotometers 
Chapter 3 Chromatography 
Section 1 Adsorption chromatography 
Section 2 Partition chromatography 
Section 3 Ion—exchange chromatography 
Section 4 Gel filtration 
Section 5 Affinity chromatography 
Chapter 4 Electrophoretic techniques 
Section 1 Basic principles 
Section 2 Several types of commonly used electrophoresis 
Chapter 5 Centrifugation techniques 
Section 1 Basic principle 
Section 2 General description of centrifugation 
Chapter 6 Genetic engineering and analytical technology of gene expression 
Section 1 Genetic engineering 
Section 2 Polymerase chain reaction 
Section 3 Nucleic acid hybridization 
Section 4 DNA sequencing technology 
Part 2 Experiments of the biochemistry and molecular biology 
Chapter 7 Analysis of purification and physicochemical properties of proteins 
Experiment 1 Colorimetric methods for protein determination 
Experiment 2 Isolation and purification of serum y—globulin 
Experiment 3 Cellulose acetate membrane electrophoresis of serum proteins 
Experiment 4 Determination of relative molecular mass of protein in SDS—PAGE 
Chapter 8 Kinetics of enzyme—catalysed reactions 
Experiment 1 The specificity of enzyme and effect of temperature, pH on the enzyme Activity 
Experiment 2 Isolation of alkaline phosphatase and deternunation ofits specific activity 
Experiment 3 Effects of substrates concentration of enzymatic activity: determination 
of Km of alkaline phosphatase 
Experiment 4 Inhibition effect ofphosphate on alkaline phosphatase 
Experiment 5 Analysis oflactic dehydrogenase isoenzyme 
Chapter 9 Substance metabolism 
Experiment 1 Extraction and identification ofliver glycogen 
Experiment 2 Determination of alanine transaminase activity (Reitman—Frankels'method) 
Experiment 3 Enzyme catalyzed transamination of amino acids detected by paper chromatography 
Chapter 10 Basic experiment of molecular biology 
Experiment 1 Preparation of the genomic DNAin the eukaryotic cells 
Experiment 2 Determination of the concentration and purity of DNA by spectroscop 
Experiment 3 Agarose gel electrophoresis ofnucleic acids 
Experiment 4 Polymerase chain reaction (PCR) 
Experiment 5 Isolation and purification of the DNA fragments 
Experiment 6 DNAligation—constructing recombinant plasmids 
Experiment 7 Preparation of E.coli competent cells 
Experiment 8 Transformation of Ca2+competent cells 
Experiment 9 The screening ofrecombinant DNA 
Experiment 10 Minipreparation ofplasmid DNA 
Experiment 11 Digestion ofplasmid DNA with restriction endonucleases 
Experiment 12 Southern blol 
Experiment 13 Expression of exogenous gene in prokaryotic cells 
Appendix Ⅰ The calculation table of ammonium sulfate saturation 
Appendix Ⅱ Common buffer solution preparation 
Appendix Ⅲ The relative molecular mass and pl ofprotem 
Appendix Ⅳ Common data ofchromatography 
Appendix Ⅴ The conversion between centrifugation speed ofcentrifugation force

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