Author: Yuefei-Xu & Yuxiang-Tian
Language: English
ISBN/ISSN: 9787030418968
Published on: 2014-09
Paperback
Language: English
ISBN/ISSN: 9787030418968
Published on: 2014-09
Paperback
《生物化学与分子生物学实验教程 Biochemistry and molecular biology laboratory course（英文版）》针对生物化学教学及研究中涉及的三类主要的生物分子——蛋白质、酶和核酸，根据生物大分子的理伦性质，结合实用的电泳技术、层次技术、离心技术、分子克隆技术等，综合系统地介绍生物大分子的分离、纯化、含量测定、分析鉴定和应用，并结合教学中物质代谢、酶促反应动力学的系统分析，设计实验并融入相关实验技术和研究新进展，使学生在实验教学中掌握知识、技能的同时，体会对科研方法的选择与评价。
Part 1 Basic techniques of biochemistry and molecular biology
Chapter 1 Basic techniques of preparation of biomacromolecules
Section 1 Salting out
Section 2 Dialysis and ultrafiltration
Chapter 2 Spectrophotometry
Section 1 Basic principles
Section 2 The basic construction of spectrophotometers
Section 3 Some home—made spectrophotometers
Chapter 3 Chromatography
Section 1 Adsorption chromatography
Section 2 Partition chromatography
Section 3 Ion—exchange chromatography
Section 4 Gel filtration
Section 5 Affinity chromatography
Chapter 4 Electrophoretic techniques
Section 1 Basic principles
Section 2 Several types of commonly used electrophoresis
Chapter 5 Centrifugation techniques
Section 1 Basic principle
Section 2 General description of centrifugation
Chapter 6 Genetic engineering and analytical technology of gene expression
Section 1 Genetic engineering
Section 2 Polymerase chain reaction
Section 3 Nucleic acid hybridization
Section 4 DNA sequencing technology
Part 2 Experiments of the biochemistry and molecular biology
Chapter 7 Analysis of purification and physicochemical properties of proteins
Experiment 1 Colorimetric methods for protein determination
Experiment 2 Isolation and purification of serum y—globulin
Experiment 3 Cellulose acetate membrane electrophoresis of serum proteins
Experiment 4 Determination of relative molecular mass of protein in SDS—PAGE
Chapter 8 Kinetics of enzyme—catalysed reactions
Experiment 1 The specificity of enzyme and effect of temperature， pH on the enzyme Activity
Experiment 2 Isolation of alkaline phosphatase and deternunation ofits specific activity
Experiment 3 Effects of substrates concentration of enzymatic activity： determination
of Km of alkaline phosphatase
Experiment 4 Inhibition effect ofphosphate on alkaline phosphatase
Experiment 5 Analysis oflactic dehydrogenase isoenzyme
Chapter 9 Substance metabolism
Experiment 1 Extraction and identification ofliver glycogen
Experiment 2 Determination of alanine transaminase activity （Reitman—Frankels'method）
Experiment 3 Enzyme catalyzed transamination of amino acids detected by paper chromatography
Chapter 10 Basic experiment of molecular biology
Experiment 1 Preparation of the genomic DNAin the eukaryotic cells
Experiment 2 Determination of the concentration and purity of DNA by spectroscop
Experiment 3 Agarose gel electrophoresis ofnucleic acids
Experiment 4 Polymerase chain reaction （PCR）
Experiment 5 Isolation and purification of the DNA fragments
Experiment 6 DNAligation—constructing recombinant plasmids
Experiment 7 Preparation of E.coli competent cells
Experiment 8 Transformation of Ca2+competent cells
Experiment 9 The screening ofrecombinant DNA
Experiment 10 Minipreparation ofplasmid DNA
Experiment 11 Digestion ofplasmid DNA with restriction endonucleases
Experiment 12 Southern blol
Experiment 13 Expression of exogenous gene in prokaryotic cells
References
Appendix
Appendix Ⅰ The calculation table of ammonium sulfate saturation
Appendix Ⅱ Common buffer solution preparation
Appendix Ⅲ The relative molecular mass and pl ofprotem
Appendix Ⅳ Common data ofchromatography
Appendix Ⅴ The conversion between centrifugation speed ofcentrifugation force
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