New Antibody Microarray Tube for Cellular Localization and Signaling Pathways

新型纳米金蛋白质定位微试管芯片和计算与信号转导

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Author: Lin Wang ,Stefan Wolifl , Minghu Jiang
Language: English
ISBN/ISSN: 9787302178039
Published on: 2008-01
Hardcover

This book describes a new integrated protein biochip system including protein localization, biocomputing and signaling pathways. In the postgenome era, proteomics study for direct analysis of a group of proteins becomes more and more important. Identification of its location often provides crucial information for understanding the function or regulation of a biven protein in biological pathways. The book discusses a new microarray tube with incorporation of nuclear and cytoplasmic protein purification and biocomputing technologies, intended for setting-up signaling pathways and making new discoveries.

Chapter 1 Introduction
1.1Antibody Array Technologies and Applications
1.21.2 Protein Phosphorylation, Localization and Function
1.3Interaction Proteomics and Pathway Building
Chapter 2 Technologies
2.1 Materials
2.2 Methods
2.3 Silver Staining in Array Tube
Chapter 3 New Cytoplasmic and Nuclear BMP2 – Signaling Pattern in U937 Cells
3.1 Modulation of Phosphorylation and Localization in BMP2 – Treated U937 Cells
3.2 BMP2 Signaling in U937 Cells
3.3 BMP2 Signaling Model in U937 Cells
Chapter 4 New Cytoplasmic and Nuclear BMP2 – Signaling in MCF7 Cells
4.1 Modulation of Phosphorylation and Localization in BMP2 – Treated MCF7 Cells
4.2 BMP2 Signaling in MCF7 Cells
4.3 BMP2 Signaling Model in MCF7 Cells
Chapter 5 New Cytoplasmic and Nuclear STI571 Signaling in K562 Cells
5.1 Modulation of Phosphorylation and Localization in STI571 – Treated K562 Cells
5.2 STI571 Signaling in K562 Cells
5.3 STI571 Signaling Model in K562 Cells
Chapter 6 Positive and Negative Co – localized Relationships in Three Treated Cell Lines
6.1Positive and Negative Co – Localized Distributions
6.2Possible Relationships
Chapter 7 Cytoplasmic Signaling in Three Treated Cell Lines
7.1 Cytoplasmic Distribution
7.2 Co-cytoplasmic Signaling
Chapter 8 Proteomic Silencing in Treated Human Cancer Cell Lines
8.1 Proteomic Silence Distributions
8.2 Possible Relationships
Chapter 9 Signaling Pathway Setting – Up Based on Bioinformatics
9.1 Methods
9.2 Results
9.3 Discussion
9.4 Conclusions
References


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